LAD1 Mouse Monoclonal Antibody [Clone ID: OTI7H8]

CAT#: CF811683

Carrier-free (BSA/glycerol-free) LAD1 mouse monoclonal antibody,clone OTI7H8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAD1 (RC213031, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAD1 (1:2000). Positive lysates LY401704 (100ug) and LC401704 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-LAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811683) (1:500)

Show all 8 images

Specifications

Product Data

• Clone Name: OTI7H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 271-517 of human LAD1 (NP_005549) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ladinin 1 (LAD1), mRNA.
• Database Link:
  NP_005549
  Entrez Gene 3898 Human
  UniProt ID O00515
• Background: The protein encoded by this gene may be an anchoring filament that is a component of basement membranes. It may contribute to the stability of the association of the epithelial layers with the underlying mesenchyme. [provided by RefSeq, Jul 2008]
• Synonyms: LadA

Reference Data

• Protein Families: Secreted Protein