PDE3B Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: CF811688

Carrier-free (BSA/glycerol-free) PDE3B mouse monoclonal antibody,clone OTI3F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE3B (RC219619, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE3B. Positive lysates LY424435 (100ug) and LC424435 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3F5
• Applications: WB
• Recommended Dilution: WB 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 654-1073 of human PDE3B (NP_000913) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124.2 kDa
• Gene Name: Homo sapiens phosphodiesterase 3B (PDE3B), transcript variant 2, mRNA.
• Database Link:
  NP_000913
  Entrez Gene 5140 Human
  UniProt ID Q13370
• Synonyms: cGIPDE1; HcGIP1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism