CDCA3 Mouse Monoclonal Antibody [Clone ID: OTI6F7]

CAT#: CF811694

Carrier-free (BSA/glycerol-free) CDCA3 mouse monoclonal antibody,clone OTI6F7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA3 (RC200821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCA3. Positive lysates LY410572 (100ug) and LC410572 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6F7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 152-252 of human CDCA3 (NP_112589) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.8 kDa
• Gene Name: Homo sapiens cell division cycle associated 3 (CDCA3), transcript variant 1, mRNA.
• Database Link:
  NP_112589
  Entrez Gene 83461 Human
  UniProt ID Q99618
• Synonyms: GRCC8; TOME-1