Dermcidin (DCD) Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: CF811720

Carrier-free (BSA/glycerol-free) DCD mouse monoclonal antibody,clone OTI3F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCD (RC209352, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCD (1:500). Positive lysates LY403291 (100ug) and LC403291 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3F5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-110 of human DCD (NP_444513) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 9.2 kDa
• Gene Name: Homo sapiens dermcidin (DCD), transcript variant 1, mRNA.
• Database Link:
  NP_444513
  Entrez Gene 117159 Human
  UniProt ID P81605
• Background: This antimicrobial gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
• Synonyms: AIDD; DCD-1; DSEP; HCAP; PIF

Reference Data

• Protein Families: Secreted Protein