Interleukin 34 (IL34) Mouse Monoclonal Antibody [Clone ID: OTI8C4]

CAT#: CF811728

Carrier-free (BSA/glycerol-free) IL34 mouse monoclonal antibody,clone OTI8C4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL34 (RC206629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL34 (1:2000). Positive lysates LY403469 (100ug) and LC403469 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IL34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811728) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-IL34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811728) (1:500)

Specifications

Product Data

• Clone Name: OTI8C4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 77-242 of human IL34 (NP_689669) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.3 kDa
• Gene Name: Homo sapiens interleukin 34 (IL34), transcript variant 1, mRNA.
• Database Link:
  NP_689669
  Entrez Gene 146433 Human
  UniProt ID Q6ZMJ4
• Background: Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]). [supplied by OMIM, May 2008]
• Synonyms: C16orf77; IL-34