HOMER1 Mouse Monoclonal Antibody [Clone ID: OTI7G10]

CAT#: CF811745

Carrier-free (BSA/glycerol-free) HOMER1 mouse monoclonal antibody,clone OTI7G10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOMER1 (RC215892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOMER1 (1:2000). Positive lysates LY418101 (100ug) and LC418101 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines and 2 different tissue lysates by using anti-HOMER1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-HOMER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811745) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-HOMER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811745) (1:500)

Specifications

Product Data

• Clone Name: OTI7G10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-354 of human HOMER1 (NP_004263) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.1 kDa
• Gene Name: Homo sapiens homer scaffold protein 1 (HOMER1), transcript variant 1, mRNA.
• Database Link:
  NP_004263
  Entrez Gene 26556 MouseEntrez Gene 29546 RatEntrez Gene 9456 Human
  UniProt ID Q86YM7
• Background: This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008]
• Synonyms: HOMER; HOMER1A; HOMER1B; HOMER1C; SYN47; Ves-1

Reference Data

• Protein Families: Druggable Genome