PDPN Mouse Monoclonal Antibody [Clone ID: OTI7B4]

CAT#: CF811746

Carrier-free (BSA/glycerol-free) PDPN mouse monoclonal antibody,clone OTI7B4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDPN (RC224811, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDPN (1:500). Positive lysates LY429296 (100ug) and LC429296 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell line by using anti-PDPN monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PDPN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811746) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDPN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811746) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-PDPN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811746) (1:500)

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  Flow cytometric analysis of living 293T cells, using anti-PDPN antibody (TA811746), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

• Flow cytometric analysis of living MCF7 cells, using anti-PDPN antibody (TA811746), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

• Flow cytometric Analysis of living U87MG cells, using anti-PDPN antibody (TA811746), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

• Flow cytometric analysis of living A431 cells, using anti-PDPN antibody (TA811746), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI7B4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 23-131 of human PDPN (NP_006465) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.7 kDa
• Gene Name: Homo sapiens podoplanin (PDPN), transcript variant 1, mRNA.
• Database Link:
  NP_006465
  Entrez Gene 10630 Human
  UniProt ID Q86YL7
• Background: This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Synonyms: AGGRUS; GP36; Gp38; GP40; HT1A-1; OTS8; PA2.26; T1A; T1A-2; T1A2; TI1A

Reference Data

• Protein Families: Druggable Genome, Transmembrane