RWDD3 Mouse Monoclonal Antibody [Clone ID: OTI8B3]

CAT#: CF811754

Carrier-free (BSA/glycerol-free) RWDD3 mouse monoclonal antibody,clone OTI8B3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RWDD3 (RC217602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RWDD3 (1:2000). Positive lysates LY414519 (100ug) and LC414519 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI8B3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-267 of human RWDD3 (NP_056300) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.3 kDa
• Gene Name: Homo sapiens RWD domain containing 3 (RWDD3), transcript variant 1, mRNA.
• Database Link:
  NP_056300
  Entrez Gene 25950 Human
  UniProt ID Q9Y3V2
• Synonyms: RSUME