METTL3 Mouse Monoclonal Antibody [Clone ID: OTI1B7]

CAT#: CF811770

Carrier-free (BSA/glycerol-free) METTL3 mouse monoclonal antibody,clone OTI1B7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METTL3 (RC200869, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METTL3 (1:2000). Positive lysates LY412720 (100ug) and LC412720 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 6 different cell lines and 3 differenet tissue lysates by using anti-METTL3 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1B7
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-284 of human METTL3 (NP_062826) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.3 kDa
• Gene Name: Homo sapiens methyltransferase like 3 (METTL3), mRNA.
• Database Link:
  NP_062826
  Entrez Gene 56335 MouseEntrez Gene 361035 RatEntrez Gene 56339 Human
  UniProt ID Q86U44
• Background: This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
• Synonyms: IME4; M6A; MT-A70; Spo8