BPOZ (ABTB1) Mouse Monoclonal Antibody [Clone ID: OTI3G8]

CAT#: CF811778

Carrier-free (BSA/glycerol-free) ABTB1 mouse monoclonal antibody,clone OTI3G8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABTB1 ([RC204306], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABTB1 (1:500).

Specifications

Product Data

• Clone Name: OTI3G8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ABTB1 (NP_115937) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.4 kDa
• Gene Name: Homo sapiens ankyrin repeat and BTB domain containing 1 (ABTB1), transcript variant 1, mRNA.
• Database Link:
  NP_115937
  Entrez Gene 80283 MouseEntrez Gene 297432 RatEntrez Gene 80325 Human
  UniProt ID Q969K4
• Background: This gene encodes a protein with an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of this gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants. [provided by RefSeq, Mar 2010]
• Synonyms: BPOZ; BTB3; BTBD21; EF1ABP; PP2259