Mps1 (TTK) Mouse Monoclonal Antibody [Clone ID: OTI1D4]

CAT#: CF811809

Carrier-free (BSA/glycerol-free) TTK mouse monoclonal antibody,clone OTI1D4

Product Images

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-TTK monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1D4
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 189-464 of human TTK (NP_003309) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.9 kDa
• Gene Name: Homo sapiens TTK protein kinase (TTK), transcript variant 1, mRNA.
• Database Link:
  NP_003309
  Entrez Gene 7272 Human
  UniProt ID P33981
• Background: This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
• Synonyms: CT96; ESK; MPH1; MPS1; MPS1L1; PYT

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway