KRT35 Mouse Monoclonal Antibody [Clone ID: OTI1A11]

CAT#: CF811820

Carrier-free (BSA/glycerol-free) KRT35 mouse monoclonal antibody,clone OTI1A11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT35 (RC218691, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT35 (1:2000). Positive lysates LY419432 (100ug) and LC419432 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KRT35 (NP_002271) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.2 kDa
• Gene Name: Homo sapiens keratin 35 (KRT35), mRNA.
• Database Link:
  NP_002271
  Entrez Gene 53617 MouseEntrez Gene 287697 RatEntrez Gene 3886 Human
  UniProt ID Q92764
• Background: The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]
• Synonyms: Ha-5; HA5; hHa5; KRTHA5