Cornulin (CRNN) Mouse Monoclonal Antibody [Clone ID: OTI4B8]

CAT#: CF811824

Carrier-free (BSA/glycerol-free) CRNN mouse monoclonal antibody,clone OTI4B8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRNN (RC204641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRNN (1:2000). Positive lysates LY414135 (100ug) and LC414135 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human esophagus within the normal limits using anti-CRNN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811824) (1:500)

Specifications

Product Data

• Clone Name: OTI4B8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRNN (NP_057274) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.4 kDa
• Gene Name: Homo sapiens cornulin (CRNN), mRNA.
• Database Link:
  NP_057274
  Entrez Gene 49860 Human
  UniProt ID Q9UBG3
• Background: This gene encodes a member of the 'fused gene' family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. The encoded protein contains two EF-hand Ca2+ binding domains in its N-terminus and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. This gene, also known as squamous epithelial heat shock protein 53, may play a role in the mucosal/epithelial immune response and epidermal differentiation. [provided by RefSeq, Jan 2009]
• Synonyms: C1orf10; DRC1; PDRC1; SEP53