Ube1L (UBA7) Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CAT#: CF811855

Carrier-free (BSA/glycerol-free) UBA7 mouse monoclonal antibody,clone OTI2B6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA7 (RC202883, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA7. Positive lysates LY418753 (100ug) and LC418753 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 cell lines by using anti-UBA7 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-UBA7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811855) (1:500)

Specifications

Product Data

• Clone Name: OTI2B6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBA7 (NP_003326) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 111.5 kDa
• Gene Name: Homo sapiens ubiquitin like modifier activating enzyme 7 (UBA7), mRNA.
• Database Link:
  NP_003326
  Entrez Gene 7318 Human
  UniProt ID P41226
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]
• Synonyms: D8; UBA1B; UBE1L; UBE2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis