MRC2 Mouse Monoclonal Antibody [Clone ID: OTI9G4]

CAT#: CF811858

Carrier-free (BSA/glycerol-free) MRC2 mouse monoclonal antibody,clone OTI9G4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRC2 (RC221360, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRC2. Positive lysates LY416912 (100ug) and LC416912 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 293T cell line by using anti-MRC2 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MRC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811858) (1:5000)

Show all 11 images

Specifications

Product Data

• Clone Name: OTI9G4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:5000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MRC2 (NP_006030) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 166.5 kDa
• Gene Name: Homo sapiens mannose receptor C type 2 (MRC2), mRNA.
• Database Link:
  NP_006030
  Entrez Gene 17534 MouseEntrez Gene 498011 RatEntrez Gene 9902 Human
  UniProt ID Q9UBG0
• Background: This gene encodes a member of the mannose receptor family of proteins that contain a fibronectin type II domain and multiple C-type lectin-like domains. The encoded protein plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. Expression of this gene may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease. [provided by RefSeq, Feb 2012]
• Synonyms: CD280; CLEC13E; ENDO180; UPARAP

Reference Data

• Protein Families: Druggable Genome, Transmembrane