ITM2A Mouse Monoclonal Antibody [Clone ID: OTI8F10]

CAT#: CF811863

Carrier-free (BSA/glycerol-free) ITM2A mouse monoclonal antibody,clone OTI8F10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2A (RC208057, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2A. Positive lysates LY401521 (100ug) and LC401521 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-ITM2A monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-ITM2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811863) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-ITM2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811863) (1:250)

Specifications

Product Data

• Clone Name: OTI8F10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:250
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ITM2A (NP_004858) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.6 kDa
• Gene Name: Homo sapiens integral membrane protein 2A (ITM2A), transcript variant 1, mRNA.
• Database Link:
  NP_004858
  Entrez Gene 9452 Human
  UniProt ID O43736
• Background: This gene encodes a type II membrane protein that belongs to the ITM2 family. Studies in mouse suggest that it may be involved in osteo- and chondrogenic differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
• Synonyms: BRICD2A; E25A

Reference Data

• Protein Families: Transmembrane