KLF5 Mouse Monoclonal Antibody [Clone ID: OTI6D2]

CAT#: CF811868

Carrier-free (BSA/glycerol-free) KLF5 mouse monoclonal antibody,clone OTI6D2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KLF5 (Cat# RC202438, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF5(Cat# TA811868). Positive lysates LY419775 (100ug) and LC419775 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-KLF5 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human spleen tissue within the normal limits using anti-KLF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811868) (1:5000)

Specifications

Product Data

• Clone Name: OTI6D2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:5000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1 - 457 of human KLF5 (NP_001721) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.6 kDa
• Gene Name: Kruppel like factor 5
• Database Link:
  NP_001721
  Entrez Gene 12224 MouseEntrez Gene 84410 RatEntrez Gene 688 Human
  UniProt ID Q13887
• Background: This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
• Synonyms: BTEB2; CKLF; IKLF

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors