UBE1C (UBA3) Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CAT#: CF811882

Carrier-free (BSA/glycerol-free) UBA3 mouse monoclonal antibody,clone OTI3F9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA3 (RC204675, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA3 (1:500). Positive lysates LY418315 (100ug) and LC418315 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from different 5 cell lines by using anti-UBA3 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI3F9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-463 of human UBA3 (NP_003959) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.7 kDa
• Gene Name: Homo sapiens ubiquitin like modifier activating enzyme 3 (UBA3), transcript variant 1, mRNA.
• Database Link:
  NP_003959
  Entrez Gene 22200 MouseEntrez Gene 117553 RatEntrez Gene 476560 DogEntrez Gene 696493 MonkeyEntrez Gene 9039 Human
  UniProt ID Q8TBC4
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: hUBA3; NAE2; UBE1C

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis