KAL1 (ANOS1) Mouse Monoclonal Antibody [Clone ID: OTI4B8]

CAT#: CF811916

Carrier-free (BSA/glycerol-free) KAL1 mouse monoclonal antibody,clone OTI4B8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KAL1 (RC212305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KAL1. Positive lysates LY400084 (100ug) and LC400084 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from MDA-MB453 cell line by using anti-KAL1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4B8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 129-373 of human KAL1(NP_000207) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 76.11 kDa
• Gene Name: Homo sapiens anosmin 1 (ANOS1), mRNA.
• Database Link:
  NP_000207
  Entrez Gene 3730 Human
  UniProt ID P23352
• Background: Mutations in this gene cause the X-linked Kallmann syndrome. The encoded protein is similar in sequence to proteins known to function in neural cell adhesion and axonal migration. In addition, this cell surface protein is N-glycosylated and may have anti-protease activity. [provided by RefSeq, Jul 2008]
• Synonyms: ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS; WFDC19