CDK15 Mouse Monoclonal Antibody [Clone ID: OTI3B9]

CAT#: CF811952

Carrier-free (BSA/glycerol-free) CDK15 mouse monoclonal antibody,clone OTI3B9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK15 (RC219623, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK15 (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CDK15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811952) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CDK15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811952) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDK15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811952) (1:500)

Specifications

Product Data

• Clone Name: OTI3B9
• Applications: IHC, WB
• Recommended Dilution: IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 293-378 of human CDK15 (NP_631897) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.4 kDa
• Gene Name: Homo sapiens cyclin dependent kinase 15 (CDK15), transcript variant 3, mRNA.
• Database Link:
  NP_631897
  Entrez Gene 271697 MouseEntrez Gene 301440 RatEntrez Gene 65061 Human
  UniProt ID Q96Q40
• Synonyms: ALS2CR7; PFTAIRE2; PFTK2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase