GIPC (GIPC1) Mouse Monoclonal Antibody [Clone ID: OTI5C10]

CAT#: CF811961

Carrier-free (BSA/glycerol-free) GIPC1 mouse monoclonal antibody,clone OTI5C10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GIPC1 (RC201175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GIPC1. Positive lysates LY404363 (100ug) and LC404363 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from Hela cell line by using anti-GIPC1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GIPC1-Knockout 293T cells (KO, Cat# LC811884) were separated by SDS-PAGE and immunoblotted with anti-GIPC1 monoclonal antibody TA811961 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-GIPC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811961) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GIPC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811961) (1:500)

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Specifications

Product Data

• Clone Name: OTI5C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GIPC1 (NP_974199) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.9 kDa
• Gene Name: Homo sapiens GIPC PDZ domain containing family member 1 (GIPC1), transcript variant 5, mRNA.
• Database Link:
  NP_974199
  Entrez Gene 67903 MouseEntrez Gene 83823 RatEntrez Gene 10755 Human
  UniProt ID O14908
• Background: GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al., 2008 [PubMed 18775991]). [supplied by OMIM, Apr 2009]
• Synonyms: C19orf3; GIPC; GLUT1CBP; Hs.6454; IIP-1; NIP; RGS19IP1; SEMCAP; SYNECTIIN; SYNECTIN; TIP-2

Reference Data

• Protein Families: Druggable Genome