DEDD Mouse Monoclonal Antibody [Clone ID: OTI7C9]

CAT#: CF811967

Carrier-free (BSA/glycerol-free) DEDD mouse monoclonal antibody,clone OTI7C9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEDD (RC212926, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEDD. Positive lysates LY409798 (100ug) and LC409798 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7C9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-318 of human DEDD (NP_127491) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.6 kDa
• Gene Name: Homo sapiens death effector domain containing (DEDD), transcript variant 1, mRNA.
• Database Link:
  NP_127491
  Entrez Gene 21945 MouseEntrez Gene 83631 RatEntrez Gene 9191 Human
  UniProt ID O75618
• Background: This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CASP8IP1; DEDD1; DEFT; FLDED1; KE05

Reference Data

• Protein Families: Druggable Genome, Transcription Factors