ARIH2 Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF811978

Carrier-free (BSA/glycerol-free) ARIH2 mouse monoclonal antibody,clone OTI1B2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARIH2 (RC201846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARIH2. Positive lysates LY416714 (100ug) and LC416714 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 cell lines by using anti-ARIH2 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 201-493 of human ARIH2 (NP_006312) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.6 kDa
• Gene Name: Homo sapiens ariadne RBR E3 ubiquitin protein ligase 2 (ARIH2), transcript variant 2, mRNA.
• Database Link:
  NP_006312
  Entrez Gene 23807 MouseEntrez Gene 316005 RatEntrez Gene 10425 Human
  UniProt ID O95376
• Background: The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]
• Synonyms: ARI2; TRIAD1

Reference Data

• Protein Families: Druggable Genome