KLF7 Mouse Monoclonal Antibody [Clone ID: OTI10D1]

CAT#: CF812007

Carrier-free (BSA/glycerol-free) KLF7 mouse monoclonal antibody,clone OTI10D1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF7 (RC215688, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF7. Positive lysates LY418489 (100ug) and LC418489 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI10D1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KLF7 (NP_003700) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.2 kDa
• Gene Name: Homo sapiens Kruppel like factor 7 (KLF7), transcript variant 1, mRNA.
• Database Link:
  NP_003700
  Entrez Gene 93691 MouseEntrez Gene 8609 Human
  UniProt ID O75840
• Background: The protein encoded by this gene is a member of the Kruppel-like transcriptional regulator family. Members in this family regulate cell proliferation, differentiation and survival and contain three C2H2 zinc fingers at the C-terminus that mediate binding to GC-rich sites. This protein may contribute to the progression of type 2 diabetes by inhibiting insulin expression and secretion in pancreatic beta-cells and by deregulating adipocytokine secretion in adipocytes. A pseudogene of this gene is located on the long arm of chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
• Synonyms: UKLF

Reference Data

• Protein Families: Transcription Factors