CREM Mouse Monoclonal Antibody [Clone ID: OTI6H4]

CAT#: CF812013

Carrier-free (BSA/glycerol-free) CREM mouse monoclonal antibody,clone OTI6H4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CREM (RC217044, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CREM. Positive lysates LY403643 (100ug) and LC403643 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6H4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CREM (NP_874388) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.3 kDa
• Gene Name: Homo sapiens cAMP responsive element modulator (CREM), transcript variant 6, mRNA.
• Database Link:
  NP_874388
  Entrez Gene 12916 MouseEntrez Gene 25620 RatEntrez Gene 1390 Human
  UniProt ID Q03060
• Background: This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]
• Synonyms: CREM-2; hCREM-2; ICER

Reference Data

• Protein Families: Druggable Genome, Transcription Factors