Alpha Fodrin (SPTAN1) Mouse Monoclonal Antibody [Clone ID: OTI9C10]

CAT#: CF812019

Carrier-free (BSA/glycerol-free) SPTAN1 mouse monoclonal antibody,clone OTI9C10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPTAN1 (RC226485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPTAN1 (1:2000).

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  Western blot analysis of extracts (35ug) from 3 cell line by using anti-SPTAN1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-SPTAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812019) (1:2000)

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Specifications

Product Data

• Clone Name: OTI9C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-147 of human SPTAN1 (NP_001123910) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant 1, mRNA.
• Database Link:
  NP_001123910
  Entrez Gene 20740 MouseEntrez Gene 64159 RatEntrez Gene 6709 Human
  UniProt ID Q13813
• Background: Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
• Synonyms: EIEE5; NEAS; SPTA2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Tight junction