GNA14 Mouse Monoclonal Antibody [Clone ID: OTI3D3]

CAT#: CF812022

Carrier-free (BSA/glycerol-free) GNA14 mouse monoclonal antibody,clone OTI3D3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNA14 (RC206547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNA14. Positive lysates LY418087 (100ug) and LC418087 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HUVEC cell line by using anti-GNA14 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GNA14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812022) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-GNA14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812022) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-GNA14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812022) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-GNA14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812022) (1:500)

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Specifications

Product Data

• Clone Name: OTI3D3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31-348 of human GNA14 (NP_004288) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.4 kDa
• Gene Name: Homo sapiens G protein subunit alpha 14 (GNA14), mRNA.
• Database Link:
  NP_004288
  Entrez Gene 14675 MouseEntrez Gene 309242 RatEntrez Gene 9630 Human
  UniProt ID O95837
• Background: This gene encodes a member of the guanine nucleotide-binding, or G protein family. G proteins are heterotrimers consisting of alpha, beta and gamma subunits. The encoded protein is a member of the alpha family of G proteins, more specifically the alpha q subfamily of G proteins. The encoded protein may play a role in pertussis-toxin resistant activation of phospholipase C-beta and its downstream effectors. [provided by RefSeq, Feb 2009]
• Synonyms: alpha 14; G alpha 14; guanine nucleotide-binding protein 14; guanine nucleotide binding protein (G protein); OTTHUMP00000021515

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway