HADHA Mouse Monoclonal Antibody [Clone ID: OTI7B3]

CAT#: CF812134

Carrier-free (BSA/glycerol-free) HADHA mouse monoclonal antibody,clone OTI7B3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADHA (RC200466, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HADHA. Positive lysates LY400065 (100ug) and LC400065 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HepG2 cell line and rat heart tissue lysate by using anti-HADHA monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and HADHA-Knockout 293T cells (KO, Cat# LC811625) were separated by SDS-PAGE and immunoblotted with anti-HADHA monoclonal antibody TA812134, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

Specifications

Product Data

• Clone Name: OTI7B3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 106-325 of human HADHA (NP_000173) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83 kDa
• Gene Name: Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000173
  Entrez Gene 97212 MouseEntrez Gene 170670 RatEntrez Gene 3030 Human
  UniProt ID P40939
• Background: This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]
• Synonyms: ECHA; GBP; HADH; LCEH; LCHAD; MTPA; TP-ALPHA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Biosynthesis of unsaturated fatty acids, Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation