KDM3A / JHDM2A (KDM3A) Mouse Monoclonal Antibody [Clone ID: OTI5A7]

CAT#: CF812138

Carrier-free (BSA/glycerol-free) KDM3A mouse monoclonal antibody,clone OTI5A7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KDM3A (RC215874, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KDM3A. Positive lysates LY402688 (100ug) and LC402688 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 2 cell lines by using anti-KDM3A monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KDM3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812138) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-KDM3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812138) (1:150)

Specifications

Product Data

• Clone Name: OTI5A7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 850-1321 of human KDM3A (NP_060903) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 147.2 kDa
• Gene Name: Homo sapiens lysine demethylase 3A (KDM3A), transcript variant 1, mRNA.
• Database Link:
  NP_060903
  Entrez Gene 104263 MouseEntrez Gene 312440 RatEntrez Gene 55818 Human
  UniProt ID Q9Y4C1
• Background: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
• Synonyms: JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA