DNAJB4 Mouse Monoclonal Antibody [Clone ID: OTI2F8]

CAT#: CF812139

Carrier-free (BSA/glycerol-free) DNAJB4 mouse monoclonal antibody,clone OTI2F8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB4 (RC203743, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB. Positive lysates LY402104 (100ug) and LC402104 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from HepG2 cell line by using anti-DNAJB4 monoclonal antibody (1:500).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and DNAJB4-Knockout HeLa cells (KO, Cat# LC833273 ) were separated by SDS-PAGE and immunoblotted with anti-DNAJB4 monoclonal antibody TA812139 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI2F8
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-337 of human DNAJB4 (NP_008965) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.6 kDa
• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member B4 (DNAJB4), transcript variant 1, mRNA.
• Database Link:
  NP_008965
  Entrez Gene 67035 MouseEntrez Gene 295549 RatEntrez Gene 11080 Human
  UniProt ID Q9UDY4
• Background: The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
• Synonyms: DjB4; DNAJW; HLJ1