ATP6V0D2 Mouse Monoclonal Antibody [Clone ID: OTI2D6]

CAT#: CF812166

Carrier-free (BSA/glycerol-free) ATP6V0D2 mouse monoclonal antibody,clone OTI2D6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V0D2 (RC209007, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP. Positive lysates LY403477 (100ug) and LC403477 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HEPG2 cell lines by using anti-ATP6V0D2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATP6V0D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812166) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ATP6V0D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812166) (1:2000)

Specifications

Product Data

• Clone Name: OTI2D6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATP6V0D2 (NP_689778) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.2 kDa
• Gene Name: Homo sapiens ATPase H+ transporting V0 subunit d2 (ATP6V0D2), mRNA.
• Database Link:
  NP_689778
  Entrez Gene 245972 Human
  UniProt ID Q8N8Y2
• Synonyms: ATP6D2; VMA6

Reference Data

• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection