Zinc Alpha 2 Glycoprotein (AZGP1) Mouse Monoclonal Antibody [Clone ID: OTI10F2]

CAT#: CF812185

Carrier-free (BSA/glycerol-free) AZGP1 mouse monoclonal antibody,clone OTI10F2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AZGP1 (RC207625, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AZGP1. Positive lysates LY420084 (100ug) and LC420084 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 tissue lysates by using anti-AZGP1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-AZGP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812185) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AZGP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812185) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AZGP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812185) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AZGP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812185) (1:2000)

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Specifications

Product Data

• Clone Name: OTI10F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-298 of human AZGP1 (NP_001176) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.1 kDa
• Gene Name: Homo sapiens alpha-2-glycoprotein 1, zinc-binding (AZGP1), mRNA.
• Database Link:
  NP_001176
  Entrez Gene 563 Human
  UniProt ID P25311
• Background: Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. [UniProtKB/Swiss-Prot Function]
• Synonyms: ZA2G; ZAG

Reference Data

• Protein Families: Secreted Protein