Bone marrow stromal cell antigen 1 (BST1) Mouse Monoclonal Antibody [Clone ID: OTI4E4]

CAT#: CF812188

Carrier-free (BSA/glycerol-free) BST1 mouse monoclonal antibody,clone OTI4E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BST1 (RC204151, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BST1. Positive lysates LY418052 (100ug) and LC418052 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E4
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-318 of human BST1 (NP_004325) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.5 kDa
• Gene Name: Homo sapiens bone marrow stromal cell antigen 1 (BST1), mRNA.
• Database Link:
  NP_004325
  Entrez Gene 683 Human
  UniProt ID Q10588
• Background: Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]
• Synonyms: CD157

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Calcium signaling pathway, Metabolic pathways, Nicotinate and nicotinamide metabolism