Cellular Apoptosis Susceptibility (CSE1L) Mouse Monoclonal Antibody [Clone ID: OTI4G1]

CAT#: CF812195

Carrier-free (BSA/glycerol-free) CSE1L mouse monoclonal antibody,clone OTI4G1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSE1L (RC211478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSE1L. Positive lysates LY420011 (100ug) and LC420011 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines by using anti-CSE1L monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CSE1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812195) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-CSE1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812195) (1:2000)

Specifications

Product Data

• Clone Name: OTI4G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-300 of human CSE1L (NP_001307) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 110.2 kDa
• Gene Name: Homo sapiens chromosome segregation 1 like (CSE1L), transcript variant 1, mRNA.
• Database Link:
  NP_001307
  Entrez Gene 110750 MouseEntrez Gene 362273 RatEntrez Gene 1434 Human
  UniProt ID P55060
• Background: Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
• Synonyms: CAS; CSE1; XPO2

Reference Data

• Protein Families: Druggable Genome