TRF1 (TERF1) Mouse Monoclonal Antibody [Clone ID: OTI3A1]

CAT#: CF812205

Carrier-free (BSA/glycerol-free) TERF1 mouse monoclonal antibody,clone OTI3A1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TERF1 (RC205904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TERF1. Positive lysates LY418833 (100ug) and LC418833 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3A1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TERF1 (NP_003209) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.1 kDa
• Gene Name: Homo sapiens telomeric repeat binding factor 1 (TERF1), transcript variant 2, mRNA.
• Database Link:
  NP_003209
  Entrez Gene 7013 Human
  UniProt ID P54274
• Background: This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
• Synonyms: hTRF1-AS; PIN2; t-TRF1; TRBF1; TRF; TRF1

Reference Data

• Protein Families: Transcription Factors