Angiotensin Converting Enzyme 1 (ACE) Mouse Monoclonal Antibody [Clone ID: OTI2G5]

CAT#: CF812212

Carrier-free (BSA/glycerol-free) ACE mouse monoclonal antibody,clone OTI2G5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE (RC223702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACE (1:500).

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  Western blot analysis of extracts (35ug) from tissue lysate by using anti-ACE monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812212) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ACE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812212) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ACE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812212) (1:500)

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Specifications

Product Data

• Clone Name: OTI2G5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 642-1230 of human ACE (NP_000780) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens angiotensin I converting enzyme (ACE), transcript variant 1, mRNA.
• Database Link:
  NP_000780
  Entrez Gene 1636 Human
  UniProt ID P12821
• Background: This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
• Synonyms: ACE1; CD143; DCP; DCP1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Hypertrophic cardiomyopathy (HCM), Renin-angiotensin system