GP210 (NUP210) Mouse Monoclonal Antibody [Clone ID: OTI7G1]

CAT#: CF812297

Carrier-free (BSA/glycerol-free) NUP210 mouse monoclonal antibody,clone OTI7G1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUP210 (RC213658, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUP21. Positive lysates LY410963 (100ug) and LC410963 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 293T cell line by using anti-NUP210 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NUP210-Knockout 293T cells (KO, Cat# LC842565 ) were separated by SDS-PAGE and immunoblotted with anti-NUP210 monoclonal antibody TA812297 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI7G1
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-400 of human NUP210 (NP_079199) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 204.9 kDa
• Gene Name: Homo sapiens nucleoporin 210 (NUP210), mRNA.
• Database Link:
  NP_079199
  Entrez Gene 23225 Human
  UniProt ID Q8TEM1
• Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]
• Synonyms: GP210; POM210

Reference Data

• Protein Families: Transmembrane