TTC7A Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF812302

Carrier-free (BSA/glycerol-free) TTC7A mouse monoclonal antibody,clone OTI2D3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTC7A (RC217177, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTC7A. Positive lysates LY412463 (100ug) and LC412463 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines by using anti-TTC7A monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-450 of human TTC7A (NP_065191) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96 kDa
• Gene Name: Homo sapiens tetratricopeptide repeat domain 7A (TTC7A), transcript variant 2, mRNA.
• Database Link:
  NP_065191
  Entrez Gene 362696 RatEntrez Gene 57217 Human
  UniProt ID Q9ULT0
• Background: This gene encodes a protein containing tetratricopeptide repeats. Mutations in this gene disrupt intestinal development and can cause early onset inflammatory bowel disease and intestinal atresia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
• Synonyms: GIDID; MINAT; TTC7