CD27 Mouse Monoclonal Antibody [Clone ID: OTI8E5]

CAT#: CF812369

Carrier-free (BSA/glycerol-free) CD27 mouse monoclonal antibody,clone OTI8E5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD27 (RC204252, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD27 (1:500).

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  Flow cytometric analysis of living 293T cells transfected with CD27 overexpression plasmid (RC204252), Red)/empty vector (PS100001, Blue) using anti-CD27 antibody (TA812369). Cells incubated with a non-specific antibody (Green) were used as isotype control.

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  Flow cytometric analysis of living Raji cells, using anti-CD27 antibody (TA812369, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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  Flow cytometric analysis of living Ramos cells, using anti-CD27 antibody (TA812369, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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  Flow cytometric analysis of living PBMCs using anti-CD27 antibody (TA812369, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI8E5
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD27 (NP_001233) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.16 kDa
• Gene Name: Homo sapiens CD27 molecule (CD27), mRNA.
• Database Link:
  NP_001233
  Entrez Gene 939 Human
  UniProt ID P26842
• Background: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProtKB/Swiss-Prot Function]
• Synonyms: S152; S152. LPFS2; T14; TNFRSF7; Tp55

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction