TNFRSF4 Mouse Monoclonal Antibody [Clone ID: OTI2B7]

CAT#: CF812371

Carrier-free (BSA/glycerol-free) TNFRSF4 mouse monoclonal antibody,clone OTI2B7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF4 (RC211253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFR. Positive lysates LY418746 (100ug) and LC418746 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC211253 overexpress plasmid (Red) or IgG isotype control (Blue) were immunostained by anti-TNFRSF4 antibody (TA812371), and then analyzed by flow cytometry (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-TNFRSF4 antibody (TA812371) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-TNFRSF4 antibody (TA812371). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI2B7
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TNFRSF4 (NP_003318) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.34 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 4 (TNFRSF4), mRNA.
• Database Link:
  NP_003318
  Entrez Gene 7293 Human
  UniProt ID P43489
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. [provided by RefSeq, Jul 2008]
• Synonyms: ACT35; CD134; IMD16; OX40; TXGP1L

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction