CD94 (KLRD1) Mouse Monoclonal Antibody [Clone ID: OTI8E10]

CAT#: CF812375

Carrier-free (BSA/glycerol-free) KLRD1 mouse monoclonal antibody,clone OTI8E10

Product Images

Flow cytometric analysis of living 293T cells transfected with KLRD1 overexpression plasmid (RC206991), Red)/empty vector (PS100001, Blue) using anti-KLRD1 antibody (TA812375). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI8E10
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-179+Mouse FC of human KLRD1 (NP_002253) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.3 kDa
• Gene Name: Homo sapiens killer cell lectin like receptor D1 (KLRD1), transcript variant 1, mRNA.
• Database Link:
  NP_002253
  Entrez Gene 3824 Human
  UniProt ID Q13241
• Background: Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CD94

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Antigen processing and presentation, Graft-versus-host disease, Natural killer cell mediated cytotoxicity