Hey L (HEYL) Mouse Monoclonal Antibody [Clone ID: OTI4D8]

CAT#: CF812473

Carrier-free (BSA/glycerol-free) HEYL mouse monoclonal antibody,clone OTI4D8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HEYL (RC202851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HEYL (1:2000). Positive lysates LY415208 (100ug) and LC415208 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4D8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HEYL (NP_055386) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.9 kDa
• Gene Name: Homo sapiens hes related family bHLH transcription factor with YRPW motif like (HEYL), mRNA.
• Database Link:
  NP_055386
  Entrez Gene 26508 Human
  UniProt ID Q9NQ87
• Background: This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: bHLHb33; HESR3; HEY3; HRT3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors