DHRS1 Mouse Monoclonal Antibody [Clone ID: OTI4C12]

CAT#: CF812489

Carrier-free (BSA/glycerol-free) DHRS1 mouse monoclonal antibody,clone OTI4C12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHRS1 (RC204206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHRS1 (1:500). Positive lysates LY408602 (100ug) and LC408602 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-DHRS1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4C12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DHRS1 (NP_612461) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens dehydrogenase/reductase 1 (DHRS1), transcript variant 2, mRNA.
• Database Link:
  NP_612461
  Entrez Gene 115817 Human
  UniProt ID Q96LJ7
• Background: This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded enzyme contains a conserved catalytic domain and likely functions as an oxidoreductase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
• Synonyms: SDR19C1

Reference Data

• Protein Families: Druggable Genome