TIM 3 (HAVCR2) Mouse Monoclonal Antibody [Clone ID: OTI12C5]

CAT#: CF812500

Carrier-free (BSA/glycerol-free) HAVCR2 mouse monoclonal antibody,clone OTI12C5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (RC209440, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAVCR. Positive lysates LY403200 (100ug) and LC403200 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of living 293T cells transfected with HAVCR2 overexpression plasmid (RC209440), Red)/empty vector (PS100001, Blue) using anti-HAVCR2 antibody (TA812500). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-HAVCR2 antibody (TA812500) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-HAVCR2 antibody (TA812500). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI12C5
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HAVCR2 (NP_116171) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.2 kDa
• Gene Name: Homo sapiens hepatitis A virus cellular receptor 2 (HAVCR2), mRNA.
• Database Link:
  NP_116171
  Entrez Gene 84868 Human
  UniProt ID Q8TDQ0
• Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
• Synonyms: CD366; HAVcr-2; KIM-3; SPTCL; Tim-3; TIM3; TIMD-3; TIMD3

Reference Data

• Protein Families: Druggable Genome, Transmembrane