LDL Receptor (LDLR) Mouse Monoclonal Antibody [Clone ID: OTI7C5]

CAT#: CF812510

Carrier-free (BSA/glycerol-free) LDLR mouse monoclonal antibody,clone OTI7C5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDLR (RC200006, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDLR (1:2000). Positive lysates LY400183 (100ug) and LC400183 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-LDLR monoclonal antibody (1:500).

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  Flow cytometric analysis of living 293T cells transfected with LDLR overexpression plasmid (RC200006), Red)/empty vector (PS100001, Blue) using anti-LDLR antibody (TA812510). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI7C5
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LDLR (NP_000518) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 95.38 kDa
• Gene Name: Homo sapiens low density lipoprotein receptor (LDLR), transcript variant 1, mRNA.
• Database Link:
  NP_000518
  Entrez Gene 3949 Human
  UniProt ID P01130
• Background: The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
• Synonyms: FH; FHC; FHCL1; LDLCQ2

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Endocytosis