Prothrombin (F2) Mouse Monoclonal Antibody [Clone ID: OTI5B11]

CAT#: CF812512

Carrier-free (BSA/glycerol-free) F2 mouse monoclonal antibody,clone OTI5B11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY F2 (RC208589, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-F2 (1:500). Positive lysates LY400182 (100ug) and LC400182 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts from human plasma, HepG2 cell line and human liver tissue by using anti-F2 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5B11
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 44-622 of human F2 (NP_000497) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.01 kDa
• Gene Name: Homo sapiens coagulation factor II, thrombin (F2), mRNA.
• Database Link:
  NP_000497
  Entrez Gene 2147 Human
  UniProt ID P00734
• Background: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
• Synonyms: PT; RPRGL2; THPH1

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton