MRP2 (ABCC2) Mouse Monoclonal Antibody [Clone ID: OTI5C3]

CAT#: CF812520

Carrier-free (BSA/glycerol-free) ABCC2 mouse monoclonal antibody,clone OTI5C3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCC2 (RC218976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCC2 (1:500).

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  Western blot analysis of extracts (35ug) from rat liver lysates by using anti-ABCC2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABCC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812520) (1:5000)

Specifications

Product Data

• Clone Name: OTI5C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:5000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1256-1545 of human ABCC2 (NP_000383) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ATP binding cassette subfamily C member 2 (ABCC2), mRNA.
• Database Link:
  NP_000383
  Entrez Gene 25303 RatEntrez Gene 1244 Human
  UniProt ID Q92887
• Background: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. [UniProtKB/Swiss-Prot Function]
• Synonyms: ABC30; CMOAT; cMRP; DJS; MRP2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters