MCF2 Mouse Monoclonal Antibody [Clone ID: OTI2C12]

CAT#: CF812536

Carrier-free (BSA/glycerol-free) MCF2 mouse monoclonal antibody,clone OTI2C12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCF2 (RC230610, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCF2 (1:500). Positive lysates LY433609 (100ug) and LC433609 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-MCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812536) (1:500)

Specifications

Product Data

• Clone Name: OTI2C12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 872-1001 of human MCF2 (NP_001093325) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens MCF.2 cell line derived transforming sequence (MCF2), transcript variant 3, mRNA.
• Database Link:
  NP_001165347
  Entrez Gene 4168 Human
  UniProt ID P10911
• Background: The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity. [provided by RefSeq, Jan 2010]
• Synonyms: ARHGEF21; DBL

Reference Data

• Protein Families: Druggable Genome