CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI1E5]

CAT#: CF812587

Carrier-free (BSA/glycerol-free) BTLA mouse monoclonal antibody,clone OTI1E5

Product Images

Flow cytometric analysis of living 293T cells transfected with BTLA overexpression plasmid (RC219458), Red)/empty vector (PS100001, Blue) using anti-BTLA antibody (TA812587). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living PBMCs, using anti-BTLA antibody (TA812587, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Specifications

Product Data

• Clone Name: OTI1E5
• Applications: FC
• Recommended Dilution: FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31-157+Mouse Fc of human BTLA (NP_861445) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: Homo sapiens B and T lymphocyte associated (BTLA), transcript variant 1, mRNA.
• Database Link:
  NP_861445
  Entrez Gene 151888 Human
  UniProt ID Q7Z6A9
• Background: This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
• Synonyms: BTLA1; CD272

Reference Data

• Protein Families: Transmembrane