MTHFR Mouse Monoclonal Antibody [Clone ID: OTI2H3]

CAT#: CF812635

Carrier-free (BSA/glycerol-free) MTHFR mouse monoclonal antibody,clone OTI2H3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTHFR (RC208588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTHFR (1:500). Positive lysates LY416960 (100ug) and LC416960 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 cell lines and 3 tissue lysates by using anti-MTHFR monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI2H3
• Applications: WB
• Recommended Dilution: WB 1:250~500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-230 of human MTHFR (NP_005948) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.4 kDa
• Gene Name: Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 2, mRNA.
• Database Link:
  NP_005948
  Entrez Gene 17769 MouseEntrez Gene 362657 RatEntrez Gene 4524 Human
  UniProt ID P42898
• Background: The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency. [provided by RefSeq, Oct 2009]
• Synonyms: 5,10-methylenetetrahydrofolate reductase (NADPH); methylenetetrahydrofolate reductase intermediate form; OTTHUMP00000002367; OTTHUMP00000002368

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Methane metabolism, One carbon pool by folate